Course Number 0452-3117-01
Course Name Methods in Molecular and Cellular Biology
Academic Unit The George S. Wise Faculty of Life Sciences -
Cell Research
Lecturer Prof. Uri AsheryContact
Lecturer Prof. Adi BarzelContact
Lecturer Prof. Gerardo LederkremerContactTo the list of all course staff
Contact Email:
Office Hours By appointmentBuilding: Sherman - Life Sciences , Room: 719
Contact Email:
Office Hours By appointmentBuilding: Building
Contact Email:
Office Hours By appointmentBuilding: CohenPorter/Britania-Life Sci , Room: 320
Mode of Instruction Lecture
Credit Hours 2
Semester 2021/1
Day Wed
Hours 12:00-14:00
Building CohenPorter/Britania-Life Sci
Room 006
Course is taught in English
Syllabus Not Found

Short Course Description

The course will cover a variety of methods for analyzing DNA, RNA, proteins and cells.
Some of the methods covered for DNA and RNA analysis are: Advanced Cloning techniques, Real time PCR, digital droplet PCR, southern blotting and northern blotting, DNA Microarrays, Next generation sequencing, single cell sequencing, In situ hybridization (FISH and CISH), Chromatin immunoprecipitation (CHIP).
For protein analysis: Pulse-chase analysis, western blotting, immunoprecipitation, determination of protein-protein interactions (FRET, BRET, pull down, split protein systems), protein complexes (isolation, 2D gels, basics of mass spectrometry and proteomics), Separation of protein complexes and organelles (subcellular fractionation, gradients).
For cell analysis and microscopy: FACS analysis, immunofluorescence, live cell imaging, confocal microscopy, TIRF, super resolution (STED, STORM), SNAP/Halo-tag, FRAP, FRET, basics of electron microscopy and fluorescence-EM correlation.

The course will also have exercises included in the lecture schedule.
Prior requirements: Cell biology, Molecular biology and biotechnology.
Mandatory presence.
Grades: There will be a final written exam. There will also be an optional assignment (30% of final grade) consisting of a short essay about a new publication on a variation of the method of a topic chosen by the student.

Full syllabus will be available to registered students only
Course Requirements

Final Exam

Students may be required to submit additional assignments
Full requirements as stated in full syllabus

The specific prerequisites of the course,
according to the study program, appears on the program page of the handbook

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